| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182374 |
| Chromosome: | chromosome 1 |
| Location: | 1514523 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007811 | (1 of 1) PF15383 - Transmembrane protein 237 (TMEM237) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGAGGGAGAGGATACGGTAGCCGGAGC |
| Internal bar code: | ACGCAACCAGAATTGCGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 106 |
| LEAP-Seq percent confirming: | 77.7448 |
| LEAP-Seq n confirming: | 262 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGCATGCTGTATTGCTTC |
| Suggested primer 2: | CAGTTCGGCTGATGCAGTAA |