| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182396 |
| Chromosome: | chromosome_11 |
| Location: | 2413908 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g475000 | IFT121 | Intraflagellar transport protein 121 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTGTGTTCGGAAAGATTCGGTGTACACTC |
| Internal bar code: | CGGCGTCATCCGTCTTACGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.7709 |
| LEAP-Seq n confirming: | 2177 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATACCGCAAGCTATGGTT |
| Suggested primer 2: | GACCGAAGGTGGCAATAGAA |