| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182397 |
| Chromosome: | chromosome 17 |
| Location: | 3506878 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725100 | (1 of 1) PTHR11390:SF20 - DNA TOPOISOMERASE 3-BETA-1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGCTGCATCCACTTCCCGCCTCACGA |
| Internal bar code: | GGAGCACCAGAAAGGTTCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 92.3729 |
| LEAP-Seq n confirming: | 436 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACATGTTCATTGTGCTCC |
| Suggested primer 2: | ACATTGTGTGGACTCGTGGA |