| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182415 |
| Chromosome: | chromosome 3 |
| Location: | 7345140 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206550 | CPLD2,PGP5 | (1 of 2) PTHR18901//PTHR18901:SF9 - 2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2 // SUBFAMILY NOT NAMED; conserved expressed protein with hydrolase motif | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGCGGCGTGGTGGAGTGTTGCGAGTG |
| Internal bar code: | GGCCTTCCGCGTCACTGCCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.8852 |
| LEAP-Seq n confirming: | 870 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGACGGATTCACGTTGT |
| Suggested primer 2: | CTGCCGCAAAGTAAAAGGAG |