| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182452 |
| Chromosome: | chromosome 12 |
| Location: | 6415990 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537700 | (1 of 1) IPR023238 - FAM175 family | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAAAATCCCACGCCCGCACACGCCCCC |
| Internal bar code: | CGACAGAACCTTCTGGCTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 68.0868 |
| LEAP-Seq n confirming: | 847 |
| LEAP-Seq n nonconfirming: | 397 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGAGTTCCGGGTTTGAAT |
| Suggested primer 2: | CCATGGGATGGTGTGTATCA |