| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182478 |
| Chromosome: | chromosome 6 |
| Location: | 7712942 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g301900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATGCGCACACGTACGTTCTTAGCCTCA |
| Internal bar code: | AATCGGCCTCGTCTGCAGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 95.5709 |
| LEAP-Seq n confirming: | 14522 |
| LEAP-Seq n nonconfirming: | 673 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGGCGTCTCTTCAAGTC |
| Suggested primer 2: | GCATGGCGGGTAGTGTAGAT |