| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182499 |
| Chromosome: | chromosome_9 |
| Location: | 6591798 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g408350 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTCTCCGTACGACCATATTGTATCGTGCC |
| Internal bar code: | CATACGTTTGCAGAGCCACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 99.2535 |
| LEAP-Seq n confirming: | 3856 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCCTGGATACCGTAGAA |
| Suggested primer 2: | GCTTCCACTCAGACTTTGCC |