| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182505 |
| Chromosome: | chromosome 8 |
| Location: | 1471430 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATACAACCCAGCTTGTTTCTCATGGTA |
| Internal bar code: | TCAGGCCAAACTCTGGGGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1242 |
| LEAP-Seq percent confirming: | 99.5361 |
| LEAP-Seq n confirming: | 2360 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCGCACCATACCTACCA |
| Suggested primer 2: | ACTCGGAAGCGAGTCACCTA |