| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182510 |
| Chromosome: | chromosome_2 |
| Location: | 751207 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g078600 | RSP8 | Radial spoke protein 8 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCGATGTGCAACGATGGCTGATTGGTTGC |
| Internal bar code: | CCGTGCGTGAACTAGCACGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.666 |
| LEAP-Seq n confirming: | 8952 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGAGAGCAAGGTCAACG |
| Suggested primer 2: | ATGTAGGAGGTGGCAAGGTG |