Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.182510 |
Chromosome: | chromosome 2 |
Location: | 751207 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g078600 | RSP8 | (1 of 239) IPR016024 - Armadillo-type fold; Radial spoke protein 8 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGATGTGCAACGATGGCTGATTGGTTGC |
Internal bar code: | CCGTGCGTGAACTAGCACGCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 930 |
LEAP-Seq percent confirming: | 99.666 |
LEAP-Seq n confirming: | 8952 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAAGGAGAGCAAGGTCAACG |
Suggested primer 2: | ATGTAGGAGGTGGCAAGGTG |