| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182531 |
| Chromosome: | scaffold_18 |
| Location: | 97676 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre18.g748697 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACGCAGTACCGCAACGCGCACAAGTACCT |
| Internal bar code: | GCTGGGCTATCTCCACCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 99.6205 |
| LEAP-Seq n confirming: | 15750 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACGCACAC |
| Suggested primer 2: | TACCCGTTTTGGTGAGAAGC |