Insertion junction: LMJ.RY0402.182537_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.182537
Chromosome:chromosome 1
Location:1019662
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g005813 POB31 Proteome of basal body 31 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGGGGGCTCGCGACCATCAACGAAACCA
Internal bar code:GGCGGTACAGAATATTTAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:518
LEAP-Seq percent confirming:98.4066
LEAP-Seq n confirming:1544
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAATAGGTCCGTCCACAG
Suggested primer 2:TCCATCTCCCTCATCTGTCC