| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182546 |
| Chromosome: | chromosome 7 |
| Location: | 2343146 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328150 | PDI4 | Protein disulfide isomerase 4; (1 of 1) PTHR18929:SF38 - PROTEIN DISULFIDE-ISOMERASE A6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTAAATCCTGTGTGTCGAGGGTAAGCA |
| Internal bar code: | TGTCGGAGCTCCACGATCCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 99.826 |
| LEAP-Seq n confirming: | 2868 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATTCGATGGTAAGCGAGT |
| Suggested primer 2: | CCTTGTCCGAACCAACTTGT |