| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182568 |
| Chromosome: | chromosome 3 |
| Location: | 5178467 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182350 | (1 of 1) K13176 - THO complex subunit 7 (THOC7) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACGTGCAGTTCCGTCTGCGCCGCCTCTC |
| Internal bar code: | TATGACCTTTCTAGCTGTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 98.6658 |
| LEAP-Seq n confirming: | 3180 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTTTAACATGGCTGCGGT |
| Suggested primer 2: | GTGCTGGGTCTCGGTATTGT |