| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182581 |
| Chromosome: | chromosome 1 |
| Location: | 6206598 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044350 | (1 of 2) PTHR11319//PTHR11319:SF28 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGTCGAGGACGTTTGGGCCGCCTTGC |
| Internal bar code: | CCCCCATCGGGCATCCCCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 98.9647 |
| LEAP-Seq n confirming: | 4875 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCTATGCCGAGCAGTCC |
| Suggested primer 2: | GTACACACAAACAGCACGGG |