| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182581 |
| Chromosome: | chromosome 1 |
| Location: | 6206602 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044350 | (1 of 2) PTHR11319//PTHR11319:SF28 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAAGCACCAAGCCATACCGTACATATAA |
| Internal bar code: | CCTGTGGTCATACGCAGGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1141 |
| LEAP-Seq percent confirming: | 97.9718 |
| LEAP-Seq n confirming: | 3816 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCTATGCCGAGCAGTCC |
| Suggested primer 2: | GTACACACAAACAGCACGGG |