Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.182584 |
Chromosome: | chromosome_12 |
Location: | 5904906 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g534050 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAGACACAGACGCGCATAAACACACACACA |
Internal bar code: | ACCGGGTAGGTGGCGACGGCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1057 |
LEAP-Seq percent confirming: | 86.619 |
LEAP-Seq n confirming: | 5211 |
LEAP-Seq n nonconfirming: | 805 |
LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCTCATAGCCCACTCGCTC |
Suggested primer 2: | GTTCACACGTGACATGAGGG |