Insertion junction: LMJ.RY0402.182590_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.182590
Chromosome:chromosome 3
Location:3841636
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g170650 (1 of 1) IPR001849//IPR011993 - Pleckstrin homology domain // Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGGTGCGGGCGACAGCACATCTACCGTA
Internal bar code:CCATCGATCAACGGTTACGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:399
LEAP-Seq percent confirming:99.9248
LEAP-Seq n confirming:1329
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GATGATGTTGAAGCGTGGTG
Suggested primer 2:GCTGCAAACTCAGAACACCA