| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182607 |
| Chromosome: | chromosome 9 |
| Location: | 6169385 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405750 | CAH8 | (1 of 2) PTHR11002//PTHR11002:SF13 - CARBONIC ANHYDRASE // BETA CARBONIC ANHYDRASE 1; Carbonic anhydrase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAATGCGGGGAAAGCTTCCTGGGCGTGT |
| Internal bar code: | GCGTCTCAATCCTTTCCACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1116 |
| LEAP-Seq percent confirming: | 51.1606 |
| LEAP-Seq n confirming: | 1631 |
| LEAP-Seq n nonconfirming: | 1557 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCTGCTATTGCCTTGCT |
| Suggested primer 2: | CCCAAGTTAACTGCGGACAT |