| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182611 |
| Chromosome: | chromosome_8 |
| Location: | 1468731 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g364600 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGGGGCTTGCTGCCGCCGCCGCCCGCCTT |
| Internal bar code: | ACCAACCACGATAACGTGTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 95.1373 |
| LEAP-Seq n confirming: | 1663 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGTCCATGTTCGATGTG |
| Suggested primer 2: | CACCTACTTCACGCACTGGA |