| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182612 |
| Chromosome: | chromosome 9 |
| Location: | 1668592 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397050 | (1 of 28) PTHR31600:SF2 - TINY MACROCYSTS PROTEIN B-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACCTGCATTGCACAGGAAGGGGGTCCT |
| Internal bar code: | GGACATAACCGCTGGGATCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 99.5937 |
| LEAP-Seq n confirming: | 4412 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGGTTGTGTGCGTGTGT |
| Suggested primer 2: | CGAATGTTGAGTGGTTGGTG |