| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182629 |
| Chromosome: | chromosome 3 |
| Location: | 3739594 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169750 | FAP371 | Flagellar Associated Protein 371 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGCGCCACGGAGGTCGACCCAAGCCCC |
| Internal bar code: | CTATTAGCTACGCTTGCTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 623 |
| LEAP-Seq percent confirming: | 98.7705 |
| LEAP-Seq n confirming: | 241 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCCCATCATCAAGTGGC |
| Suggested primer 2: | GGGATTGTAGGAGCCTGTGA |