Insertion junction: LMJ.RY0402.182651_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.182651
Chromosome:chromosome 8
Location:733094
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g360450 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGGCTGCGAGGCGTACTGCTGAAATCA
Internal bar code:TGCGTGATGTAAAAAGTTTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:370
LEAP-Seq percent confirming:32.4183
LEAP-Seq n confirming:933
LEAP-Seq n nonconfirming:1945
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TTCACGCACACAACCTAAGC
Suggested primer 2:ATCGGCTTCCTTTTTGTGTG