| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182656 |
| Chromosome: | chromosome 6 |
| Location: | 8828604 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g310450 | (1 of 1) K03124 - transcription initiation factor TFIIB (TFIIB, GTF2B, SUA7, tfb) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCAGTGCAAACAGCCAACCGAGCGCGCG |
| Internal bar code: | GGTCGTCGTTTAGCATGCTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1012 |
| LEAP-Seq percent confirming: | 94.7056 |
| LEAP-Seq n confirming: | 1914 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCGGTCAACAAGACAAG |
| Suggested primer 2: | TGTTTAAGACAGCTGGCACG |