Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.182659 |
Chromosome: | chromosome_12 |
Location: | 5723098 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g532850 | PHR3 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAGGAGACAGGACAAGGAAACAGGAAGGTG |
Internal bar code: | GTGTTCTGGATCCGACATGCAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1092 |
LEAP-Seq percent confirming: | 97.6636 |
LEAP-Seq n confirming: | 1045 |
LEAP-Seq n nonconfirming: | 25 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCCAGCTATAGAAACCGCT |
Suggested primer 2: | CATATGGGTTGATTCCCCTG |