| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182660 |
| Chromosome: | chromosome 3 |
| Location: | 5173875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182300 | PGM7 | (1 of 2) 5.4.2.11 - Phosphoglycerate mutase (2,3-diphosphoglycerate-dependent) / Phosphoglyceromutase; Phosphoglycerate mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGCAATATGTCCTCATTTCCCAGCCAT |
| Internal bar code: | ACGGCAAGAATACACTGGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 99.6651 |
| LEAP-Seq n confirming: | 2976 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCTAGGCACCTGGTTTG |
| Suggested primer 2: | TTGTCCTGACGGTAACCTCC |