| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182665 |
| Chromosome: | chromosome 3 |
| Location: | 2858517 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162650 | (1 of 3) 1.5.1.5//3.5.4.9 - Methylenetetrahydrofolate dehydrogenase (NADP(+)) // Methenyltetrahydrofolate cyclohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTGAACCCAAAAAACGGCATTGAAACG |
| Internal bar code: | ATGTTCGAGAGAGTGCAGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 921 |
| LEAP-Seq percent confirming: | 98.3738 |
| LEAP-Seq n confirming: | 4537 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTATGTGCTGCGTGTGTG |
| Suggested primer 2: | GCCCAACCATTGTCAGCTAT |