Insertion junction: LMJ.RY0402.182676_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.182676
Chromosome:chromosome 6
Location:783820
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g254100 HRP6,HGRP1 (1 of 80) IPR003882 - Pistil-specific extensin-like protein; Hydroxyproline-rich glycoprotein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAATTCAGACGGGATGCAATGATGCATAC
Internal bar code:AGGGATGGTGGCTTTGTGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:452
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:80
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TAATGCAATGTTTACGGGCA
Suggested primer 2:AGGTGTGTTCCGAGTTGACC