| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182702 |
| Chromosome: | chromosome_2 |
| Location: | 7353041 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g145100 | FAP39 | P-type ATPase/cation transporter, flagellar associated | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCGAGCCGGCCCAGCCAGCCGACCGGGGG |
| Internal bar code: | TATTTTTGGCTATTAACAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 99.5841 |
| LEAP-Seq n confirming: | 7184 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAAACGCGGTAGGTGACG |
| Suggested primer 2: | GTTCAGCTTCTGGAACTGCC |