| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182705 |
| Chromosome: | chromosome 9 |
| Location: | 7590958 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414626 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGCTTGTACGACGCCGATGAGGCGGAC |
| Internal bar code: | CTGCTGCTTTGGGTCGGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1263 |
| LEAP-Seq percent confirming: | 99.1612 |
| LEAP-Seq n confirming: | 2128 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTAATACGCTCTTCGCGG |
| Suggested primer 2: | CCTTCCAAGAACGCTTTGAG |