| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182713 |
| Chromosome: | chromosome 16 |
| Location: | 2157544 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658200 | (1 of 2) IPR003594//IPR024975 - Histidine kinase-like ATPase, C-terminal domain // Domain of unknown function DUF3883 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTAACCTCTGGCCCTGCCCCACCCCACAC |
| Internal bar code: | TCTCCTTGACAACGGATAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 95.8886 |
| LEAP-Seq n confirming: | 1446 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTTGTCCTCACACCCGAC |
| Suggested primer 2: | TGGCACTCACACTTCCTCAG |