| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182737 |
| Chromosome: | chromosome 7 |
| Location: | 211471 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313650 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGCCCAACCCCAGCCCCAGGCCCGAAC |
| Internal bar code: | TCCGGAGGCGGCCGGACAATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 99.6717 |
| LEAP-Seq n confirming: | 2732 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACTTGGGTGGAAGACT |
| Suggested primer 2: | GCAAACTCACAAGCTCACCA |