Insertion junction: LMJ.RY0402.182739_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.182739
Chromosome:chromosome 2
Location:3500208
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095125 (1 of 1) IPR000104//IPR000408//IPR009091 - Antifreeze protein, type I // Regulator of chromosome condensation, RCC1 // Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCTCGGTCCGACGCACCGCCATACCTA
Internal bar code:TAGGTTGATCTTGGGATGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1027
LEAP-Seq percent confirming:98.7879
LEAP-Seq n confirming:5379
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTACTGGAACACGGACG
Suggested primer 2:AGGGTTTTCACCATACGCAC