Insertion junction: LMJ.RY0402.182751_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.182751
Chromosome:chromosome 5
Location:289155
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g233550 CBC1 (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAACCGACCAAACAGCACATTCCATTAGG
Internal bar code:AGGTGGGTATGCTCGTGGATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:878
LEAP-Seq percent confirming:99.1485
LEAP-Seq n confirming:145782
LEAP-Seq n nonconfirming:1252
LEAP-Seq n unique pos:317

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCGGTTTGGTTTGTATT
Suggested primer 2:ACATTAGGGTTTCAGGTGCG