Insertion junction: LMJ.RY0402.182756_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.182756
Chromosome:chromosome 1
Location:5059794
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g035000 (1 of 1) K14791 - periodic tryptophan protein 1 (PWP1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGATCAACTTCGATCCCCCGACTTGAGAC
Internal bar code:TGCCAGTTCCGGGCGATCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:861
LEAP-Seq percent confirming:99.5989
LEAP-Seq n confirming:1738
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGGCTCAAGAGTCAAACC
Suggested primer 2:CCTTCTTGTCGCTCTTGTCC