| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182759 |
| Chromosome: | chromosome 3 |
| Location: | 2809213 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162300 | SDR7 | Short-chain dehydrogenase/reductase; (1 of 13) 1.1.1.300 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGTTCAGCCGCTGGCCGGCCGCACACA |
| Internal bar code: | GCCAATTCATCGGAGTGCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 97.8758 |
| LEAP-Seq n confirming: | 599 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACACGTCGATCTGTGTGG |
| Suggested primer 2: | CGTGTGGTGTGTGTGTGTGT |