| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182761 |
| Chromosome: | chromosome 7 |
| Location: | 5562449 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351550 | (1 of 1) PTHR31319//PTHR31319:SF1 - FAMILY NOT NAMED // CCT MOTIF FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCACATCGCCCTGGCCGTAACTCGTGCG |
| Internal bar code: | CCCGGACACTATTTGGGTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 99.6071 |
| LEAP-Seq n confirming: | 4310 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTGCAATCACTCGGCAA |
| Suggested primer 2: | AGCAACAAAACACACCACCA |