| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182763 |
| Chromosome: | chromosome 12 |
| Location: | 7006690 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559850 | (1 of 1) K12880 - THO complex subunit 3 (THOC3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTTTTGATATGTAGCTGAACACAACGT |
| Internal bar code: | TAACTTGGGCAATCTGATAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 513 |
| LEAP-Seq percent confirming: | 98.8876 |
| LEAP-Seq n confirming: | 1689 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGGAGGAGGTCATCTGC |
| Suggested primer 2: | CAATTGTCAACAGCAGCGAT |