| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182763 |
| Chromosome: | chromosome 12 |
| Location: | 7006695 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559850 | (1 of 1) K12880 - THO complex subunit 3 (THOC3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACATCCCCTGCAAAGCTGCAGCCCAGTAG |
| Internal bar code: | TTCAGGCTCGAACCTCCAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 988 |
| LEAP-Seq percent confirming: | 99.4877 |
| LEAP-Seq n confirming: | 3884 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATTGTCAACAGCAGCGAT |
| Suggested primer 2: | ACATGGAGGAGGTCATCTGC |