| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182764 |
| Chromosome: | chromosome_5 |
| Location: | 3038732 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g238650 | PHC5 | Cell wall protein pherophorin-C5 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGGCCGCAGCTGCTCGGCAGGCCCTACCC |
| Internal bar code: | GAGGGTTTACAGGCCTATTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 99.6198 |
| LEAP-Seq n confirming: | 524 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCATCAGCTAGTAAAGCG |
| Suggested primer 2: | AAACATAAATCTGCCCAGCG |