| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182771 |
| Chromosome: | chromosome 11 |
| Location: | 1440287 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467730 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | 3'UTR_intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCCCTGTGCCTCAGGGGCAAAGTTAAC |
| Internal bar code: | AGCAACTGGTTTTAGAACGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 99.898 |
| LEAP-Seq n confirming: | 979 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGCGAAGACATCTGAGG |
| Suggested primer 2: | CTGGCGTAGCAGTGTGTGAT |