| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182778 |
| Chromosome: | chromosome 1 |
| Location: | 2374601 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013000 | (1 of 1) 2.7.7.31 - DNA nucleotidylexotransferase / Terminal transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGCAGCCGCAGACATTGTCACAGCGGG |
| Internal bar code: | CGGACTGGAGTTGCAGTGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 22.9147 |
| LEAP-Seq n confirming: | 728 |
| LEAP-Seq n nonconfirming: | 2449 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGTCGTACGGTTGTGGT |
| Suggested primer 2: | CACAGCAAAATGGTGTTTGG |