| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182779 |
| Chromosome: | chromosome 14 |
| Location: | 2041241 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621800 | GT90-27,GT90F27 | GT90 family protein 27; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTCGGTAACGGCGGGCGAGGCGCTGTG |
| Internal bar code: | CACGATGGGCGCGGCGATTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 99.9387 |
| LEAP-Seq n confirming: | 1629 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAAAGATTTCACCAGCG |
| Suggested primer 2: | GGTACTTCCCGTTCCTGACA |