| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182780 |
| Chromosome: | chromosome 12 |
| Location: | 1209287 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGAATCAATGCATGAAACGCGTCAACG |
| Internal bar code: | CGGTGGCAGTGGCAGGGATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 994 |
| LEAP-Seq percent confirming: | 86.641 |
| LEAP-Seq n confirming: | 4942 |
| LEAP-Seq n nonconfirming: | 762 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGTGTGATGTGTGGGCT |
| Suggested primer 2: | CGACTGGGGACAGAAGTCAT |