| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182794 |
| Chromosome: | chromosome 5 |
| Location: | 899498 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247450 | CGL56,RDP5 | Putative rhodanese-like protein; (1 of 1) PTHR34209//PTHR34209:SF1 - FAMILY NOT NAMED // RHODANESE/CELL CYCLE CONTROL PHOSPHATASE SUPERFAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGATGCGTGTAGGGCGACGCCGCAAG |
| Internal bar code: | TTCCTTGACTTGGCCCTCTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 99.8164 |
| LEAP-Seq n confirming: | 2718 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTAAGGAGGCTTTGTGG |
| Suggested primer 2: | CAGCGTCCCGACATTAAGTT |