| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182796 |
| Chromosome: | chromosome 12 |
| Location: | 4158573 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518300 | SPL25 | Pre-mRNA splicing factor; (1 of 1) K12871 - coiled-coil domain-containing protein 12 (CCDC12) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCACCATTGCAGCCACGTGCATGCAGCC |
| Internal bar code: | GCACCGAGTGGGGATTGAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 99.3916 |
| LEAP-Seq n confirming: | 1307 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGGTTGCTACGAAGGCT |
| Suggested primer 2: | TATTGCTGCTTAGGGCTCGT |