Insertion junction: LMJ.RY0402.182806_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.182806
Chromosome:chromosome 3
Location:8046247
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g201400 PPP16,PP2C6 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAAAGCCTGCGGACACATGGACGGAGTG
Internal bar code:GAGGAGGCCGTTGAGCGAAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1114
LEAP-Seq percent confirming:99.8472
LEAP-Seq n confirming:2613
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCCCTTCTTCTTCTTTTT
Suggested primer 2:ATGCCTACATACCAGCGGAG