| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182816 |
| Chromosome: | chromosome 3 |
| Location: | 6029097 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191100 | (1 of 1) PF15016 - Domain of unknown function (DUF4520) (DUF4520) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTCGCAGAGGCCCAACGCCCGAGTTTGA |
| Internal bar code: | GAGCTGGGCCGAGTAGGTCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 99.6655 |
| LEAP-Seq n confirming: | 3575 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACTCGTACTTGGCCTCC |
| Suggested primer 2: | CGTCCAAGGAAGATGAAGGA |