Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.182852 |
Chromosome: | chromosome_10 |
Location: | 4127896 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g449750 | PKS1 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGTCGCACCCGCGCCTGGCCCACCTGTGG |
Internal bar code: | GGGGCACTTCAGGGACGTCGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 779 |
LEAP-Seq percent confirming: | 93.8995 |
LEAP-Seq n confirming: | 1570 |
LEAP-Seq n nonconfirming: | 102 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCGCTGTGAACCTTCATCC |
Suggested primer 2: | CAGGAAGCAGTCCAGTAGCC |