| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182857 |
| Chromosome: | chromosome 17 |
| Location: | 3571580 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725550 | GLD1 | Glucose-6-phosphate-1-dehydrogenase; (1 of 2) 1.1.1.49 - Glucose-6-phosphate dehydrogenase (NADP(+)) / GPD | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGTATGGCTAAAACGAGTCACTCCCC |
| Internal bar code: | TGAGGCTAGGTAATCATCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 96.6619 |
| LEAP-Seq n confirming: | 7500 |
| LEAP-Seq n nonconfirming: | 259 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGAGGCCCAACAATGAAG |
| Suggested primer 2: | ACCTTGAAGCAGAAGTCCGA |