| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182868 |
| Chromosome: | chromosome 9 |
| Location: | 1752831 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396500 | HAD3 | Halo-acid dehalogenase-like hydrolase; (1 of 98) IPR023214 - HAD-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGGCGAGGGCGTGGAGGAGTCGGGGAA |
| Internal bar code: | GGATATTGATGGCCTGCGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 984 |
| LEAP-Seq percent confirming: | 99.0084 |
| LEAP-Seq n confirming: | 1298 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGGGGAGAGGGTAGATA |
| Suggested primer 2: | ACCACCCACTCCACTCACTC |